Composite

Part:BBa_K4595021:Design

Designed by: Tang rui   Group: iGEM23_HUST-China   (2023-10-11)


Prbcl-gshA-gshB-Tpsbc


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 145
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 145
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 145
    Illegal BamHI site found at 1210
    Illegal BamHI site found at 1683
    Illegal BamHI site found at 2187
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 145
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 145
    Illegal NgoMIV site found at 2164
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We codon-optimized its sequence.


Source

Synechocystis PCC6803

References